In the Cell and Tissue Engineering Laboratory at the I.R.C.C.S Galeazzi Orthopedic Institute, Dr. Matteo Moretti uses a SONICA ultrasonic cleaner to wash and decontaminate the polymeric material growth rooms.

Below is described how the ultrasonic cleaner and the SONICA CL4% disinfectant soap are used.

Bioreactor prototypes for tissue engineering are developed in this laboratory. One day they will permit the creation in vitro of functional biological tissue that could be directly implanted in human beings.

These prototypes use growth rooms made of polymeric material in which three dimensional holders called scaffolds are placed. First of all, the cells are seed on the scaffolds and then they are stimulated to produce extracellular matrix through interstitial perfusion in a bioreactor.

During these research the growth rooms in which the tests are performed are used several times. It is therefore necessary to make sure that there are no biological residuals in the rooms before the autoclave sterilisation process. In addition, a manual cleaning process would not guarantee the same efficiency.

The ultrasonic cleaner SONICA 2200MH together with SONICA CL4% detergent allows the cleaning and decontamination of the growth rooms. It is only necessary to rinse them thoroughly with demineralised water before the sterilisation process.

All the information above regarding the cleaning and decontamination process of the bioreactor prototypes performed with SONICA have been released by:

Matteo Moretti, Ph.D.
Head of Cell and Tissue Engineering Laboratory,
I.R.C.C.S. Istituto Ortopedico Galeazzi

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